| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068072 |
| Chromosome: | chromosome_16 |
| Location: | 5549562 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g680250 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGTAAACTCCCACATTGCCCTTCATGTCG |
| Internal bar code: | TTACACCATGATATACGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.2576 |
| LEAP-Seq n confirming: | 5348 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGGCGTGCACAGACAG |
| Suggested primer 2: | TTGTATTACCGTTCTCCCCG |