| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.068110 |
| Chromosome: | chromosome 2 |
| Location: | 7462234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144251 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCAAACCCCACCGACCTTGAGGCCGTC |
| Internal bar code: | GCAATCCCCAGCTTTCACCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 88.7356 |
| LEAP-Seq n confirming: | 1930 |
| LEAP-Seq n nonconfirming: | 245 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACACCCTCTCC |
| Suggested primer 2: | ACTGTTTCCTCACGGGAATG |