| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068143 |
| Chromosome: | chromosome 3 |
| Location: | 6320094 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193450 | (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCTGCCGGATGTCGAGTTTGGCAGGCA |
| Internal bar code: | TGGCGAGTTGGTGGAAAGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 99.2434 |
| LEAP-Seq n confirming: | 5378 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGCTAGCACTCTTGTAA |
| Suggested primer 2: | GCGCGTCACTGATAGCATTA |