| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068149 |
| Chromosome: | chromosome 17 |
| Location: | 51314 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696500 | PHC19 | Pherophorin-chlamydomonas homolog 19; (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGCTCTGTGTCCGGAGGGAGATGGCA |
| Internal bar code: | GTATCCCTGAATGGGGGGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 252 |
| LEAP-Seq percent confirming: | 75.5228 |
| LEAP-Seq n confirming: | 9895 |
| LEAP-Seq n nonconfirming: | 3207 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGACGGGCCTTTAAACT |
| Suggested primer 2: | GGGTACTGCACCTTGTCGAT |