Insertion junction: LMJ.RY0402.068173_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.068173
Chromosome:chromosome_6
Location:7927498
Confidence (%):73
Locus disrupted Locus common name Defline Orientation Feature
Cre06.g303400 FAP16 Flagellar Associated Protein sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TGCGAGTGGAGGGCGCACACCGATGTAGGG
Internal bar code:TTGTCATGGGCTCATGAGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:218
LEAP-Seq percent confirming:86.8709
LEAP-Seq n confirming:397
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTCAAGTTCATGAGCGA
Suggested primer 2:CTCCTCTGTTCAAAGCCGAC