| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068231 |
| Chromosome: | chromosome 14 |
| Location: | 2823434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627433 | (1 of 1) PF04142 - Nucleotide-sugar transporter (Nuc_sug_transp) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGATATATGACACGAAGGCATCACTGTG |
| Internal bar code: | TACGTCGGGTTTTAGCCGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 92.8872 |
| LEAP-Seq n confirming: | 5511 |
| LEAP-Seq n nonconfirming: | 422 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGCCTGCTTGAAGTGAT |
| Suggested primer 2: | TGTAGTGTGTTCCCGGACAA |