| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.068254 |
| Chromosome: | chromosome 11 |
| Location: | 1824675 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467650 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAATCACGTCAGTACGAGGAGCGTCCTT |
| Internal bar code: | ATTCAACAGACTCGACGATTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 99.0793 |
| LEAP-Seq n confirming: | 1399 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTATTACGTGGTGCCT |
| Suggested primer 2: | CTAAGCACACGCCCCTATGT |