| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068292 |
| Chromosome: | chromosome 16 |
| Location: | 6814345 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674852 | HAP2,GCS1 | (1 of 1) PF10699 - Male gamete fusion factor (HAP2-GCS1); Generative cell specific-1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATGCAGGCTCTATGCGCTCTGCTCCCT |
| Internal bar code: | GTCTCGTGGGCAGATGTCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 83 |
| LEAP-Seq percent confirming: | 99.7207 |
| LEAP-Seq n confirming: | 357 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTAGCATCTTCTTCAGC |
| Suggested primer 2: | TGGGTGTTTGGCTAGGTAGG |