Insertion junction: LMJ.RY0402.068314_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.068314
Chromosome:chromosome 17
Location:1591538
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g707700 NRM1,DMT1,NRAMP1 (1 of 2) PTHR11706:SF33 - NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1; Manganese/metal transporter, NRAMP homolog intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCCATCCCGATTACCTGAAGGAACATGG
Internal bar code:CAGTCACATTTGGGTTGGCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:672
LEAP-Seq percent confirming:99.256
LEAP-Seq n confirming:2935
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTTGCGGTAACGGTGAT
Suggested primer 2:CAGCGCCAAGATACAAGTCA