| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068326 |
| Chromosome: | chromosome 17 |
| Location: | 1796741 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709500 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGTGTAGCCACCGAGCTGGATGCGCG |
| Internal bar code: | CTCGAGGGTTATTGTCACTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 90.5941 |
| LEAP-Seq n confirming: | 183 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCACGTAGCTTGTGAGC |
| Suggested primer 2: | ATGTAGCCATCAAGGCGTTC |