| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068333 |
| Chromosome: | chromosome 3 |
| Location: | 7278762 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207200 | (1 of 18) PF00650 - CRAL/TRIO domain (CRAL_TRIO) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCTCCCACGCCTGGTACAGGCATCTC |
| Internal bar code: | GGTTTGGCGACGTGGGGTACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.7667 |
| LEAP-Seq n confirming: | 8980 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATGACCCAAGCGATGAGG |
| Suggested primer 2: | AATGATGGCCTCTATGCAGC |