| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068339 |
| Chromosome: | chromosome 6 |
| Location: | 4885879 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279050 | (1 of 7) PTHR11584:SF381 - PROTEIN PQN-80, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCGGCGTCCTCCTGTGCGTCCATCACGA |
| Internal bar code: | GGGTGCAGTAACGCAGGGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 94.4751 |
| LEAP-Seq n confirming: | 171 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTATTGCTCACAAGTCCA |
| Suggested primer 2: | CAAGACCGTCAAAGCACTCA |