| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068379 |
| Chromosome: | chromosome 12 |
| Location: | 9047289 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543301 | CSV11 | Chlamydomonas-specific family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGAAGCTGGCATGCATGGAGTGCGGCA |
| Internal bar code: | CGCAGGTTATGAGTACAGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1285 |
| LEAP-Seq percent confirming: | 99.7455 |
| LEAP-Seq n confirming: | 8230 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAAAACTTCAGGCAGGC |
| Suggested primer 2: | GTTGCTGTTGCTGTTGCTGT |