| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068404 |
| Chromosome: | chromosome 1 |
| Location: | 2103296 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g011350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATCCCGCGCGGCACGCCCGGCGCGCCCC |
| Internal bar code: | AACGGATTACGGGCGACGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 869 |
| LEAP-Seq percent confirming: | 99.2778 |
| LEAP-Seq n confirming: | 6461 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATCTTCATCAGCGTGTG |
| Suggested primer 2: | CAGCATACCGTGTGTCCTTG |