| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068425 |
| Chromosome: | chromosome 16 |
| Location: | 3740239 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689100 | PUS6 | (1 of 2) 5.4.99.23 - 23S rRNA pseudouridine(1911/1915/1917) synthase; RNA pseudouridine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCACCTAACCCCGCGACCGTATCACCA |
| Internal bar code: | GCTTACCGATGTAATCTCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1256 |
| LEAP-Seq percent confirming: | 99.718 |
| LEAP-Seq n confirming: | 2475 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCCATTGTGCTGTCCT |
| Suggested primer 2: | CAGCCTCACTCCCTACTTCG |