| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068431 |
| Chromosome: | chromosome 12 |
| Location: | 6946230 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560400 | DNJ16 | (1 of 1) PTHR24078:SF319 - DNAJ HOMOLOG SUBFAMILY C MEMBER 16; DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAAGAAAGTGGGCCGTTGCAGGCAGGTT |
| Internal bar code: | CGGGCCCTGCGTTTCCTGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 99.3545 |
| LEAP-Seq n confirming: | 1693 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAATATGGGATGGACGTG |
| Suggested primer 2: | GGAGAAAGGAGCTTGTGTCG |