Insertion junction: LMJ.RY0402.068438_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.068438
Chromosome:chromosome 12
Location:8422805
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g548550 (1 of 1) PF05742 - Transport and Golgi organisation 2 (TANGO2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTCGCGGTTGAAGGCGAGCAGGAAACGA
Internal bar code:ACGCACTCGCTGTAGATGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1126
LEAP-Seq percent confirming:99.2439
LEAP-Seq n confirming:5250
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACTGTGTGCAATGGACC
Suggested primer 2:GCGTCCAGAGACTGTGACAA