Insertion junction: LMJ.RY0402.068447_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.068447
Chromosome:chromosome 1
Location:4376987
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g029600 (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTTTCAATGTGGAAGGTGGATGATGGAA
Internal bar code:CACCACGCGGAACGGGTACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:319
LEAP-Seq percent confirming:99.8802
LEAP-Seq n confirming:1668
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCGACTACATGGACAAGC
Suggested primer 2:TAAGGTATGCCCCTCTCTGC