| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068459 |
| Chromosome: | chromosome 3 |
| Location: | 4102887 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACACCTCTGCTTCATACCGGCCAGGCT |
| Internal bar code: | GCGGGAAATTTCAAGTTAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 95.069 |
| LEAP-Seq n confirming: | 482 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCAAAGGGACAGAAGAG |
| Suggested primer 2: | TGGAGTGGAGTGGTGTGGTA |