| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068459 |
| Chromosome: | chromosome_17 |
| Location: | 2894767 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g719600 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAATCATAGAAGTGCTGTCTCCAAACAAA |
| Internal bar code: | GGGAGAGAACAAAGAGGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 984 |
| LEAP-Seq percent confirming: | 77.499 |
| LEAP-Seq n confirming: | 1946 |
| LEAP-Seq n nonconfirming: | 565 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCGAAGTTAAGTGCGGT |
| Suggested primer 2: | ACGACGTGTCCCTAAAGGTG |