| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068459 |
| Chromosome: | chromosome_17 |
| Location: | 2894925 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g719600 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCGACAGCCCCGAGGTAGTTACGTAGAG |
| Internal bar code: | GTACTCGGCGGTGTATTCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 99.7481 |
| LEAP-Seq n confirming: | 14254 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTCAGCAGTTAGCCTC |
| Suggested primer 2: | TGCAGTGAAGCCGTATCAAG |