| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068463 |
| Chromosome: | chromosome 17 |
| Location: | 1519965 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707200 | LIC2 | (1 of 5) PF02931 - Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD); Ligand-gated ion channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTGAAGGCAAGCAGGGGGCAAGCGGAA |
| Internal bar code: | GCTGTGTCCAGGGGGTACCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 98.698 |
| LEAP-Seq n confirming: | 6216 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACCACACACACCTGA |
| Suggested primer 2: | TTTCCCACCACCTACTGCTC |