| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068467 |
| Chromosome: | chromosome 7 |
| Location: | 4602236 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCGCCTCCGGTGCCGGCGGTGCTGGG |
| Internal bar code: | GTTGACGCAAAAATGCCTGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 58 |
| LEAP-Seq percent confirming: | 97.4026 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTTTGTCCTCGTGCTCA |
| Suggested primer 2: | TGAGAAAGTGAGGATGTGCG |