Insertion junction: LMJ.RY0402.068480_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.068480
Chromosome:chromosome 6
Location:7363824
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g299150 PHC9,PHC26 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 26 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGAATTCTTTGCCGGTGAGTAGGGAATG
Internal bar code:GCTCTTAAAGGGCCATGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:672
LEAP-Seq percent confirming:96.4286
LEAP-Seq n confirming:486
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCAAACACAAAGAGGGG
Suggested primer 2:CAACACTCCACCAACACCAG