| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.068503 |
| Chromosome: | chromosome 2 |
| Location: | 4679454 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101550 | MRS1 | BTB/POZ domain protein; (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAAGAGCGCGGCGGCCCGCCGTCTGCG |
| Internal bar code: | GCACGACGCAGCAGGGTGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 83.6842 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAAGTAGTCCTGGTGCC |
| Suggested primer 2: | TGTCTGTATCTGGCGGACTG |