Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.068514 |
Chromosome: | chromosome 14 |
Location: | 1904202 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g620850 | NSG17 | (1 of 9) PF00010 - Helix-loop-helix DNA-binding domain (HLH); Basic helix-loop-helix transcription factor | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAAACACGGGTTTGCGGAGCCCCGCCC |
Internal bar code: | TGATCCTAAGTTGTAAGCCACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1218 |
LEAP-Seq percent confirming: | 99.7561 |
LEAP-Seq n confirming: | 6135 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGAAGTCAGGGGAAAAGG |
Suggested primer 2: | TTGAGCAGTGGTCTGGACTG |