| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068517 |
| Chromosome: | chromosome 2 |
| Location: | 3470786 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095120 | ANK18 | Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGGAGCAGTAAAGGCGAGTCCCGGATG |
| Internal bar code: | CGAGACACGCAGGATGCCCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 99.6647 |
| LEAP-Seq n confirming: | 16644 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTGGGTGTAATCGGCTG |
| Suggested primer 2: | CAGCACTACATGGAGGAGCA |