| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068553 |
| Chromosome: | chromosome 12 |
| Location: | 4880872 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525200 | NOP56 | (1 of 1) K14564 - nucleolar protein 56 (NOP56); Nucleolar protein, Component of C/D snoRNPs | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTATCACCCCCGCGGACCACCTCAGCAAC |
| Internal bar code: | AAGGATAGGTATCGGGGCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 903 |
| LEAP-Seq percent confirming: | 99.5417 |
| LEAP-Seq n confirming: | 3475 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCGCGTATTCATACCTT |
| Suggested primer 2: | CTACCTGGCCAACAAGTGCT |