| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.068558 |
| Chromosome: | chromosome 8 |
| Location: | 209803 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358547 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGTTACACAATGCCCTCAAATGGCGCT |
| Internal bar code: | AGGGTTAGAGTCAGTGACCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 94.4861 |
| LEAP-Seq n confirming: | 1765 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGTCAAGGAGGGAGCAG |
| Suggested primer 2: | ATGCGTGCTACGTGTCAAAG |