| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068576 |
| Chromosome: | chromosome 6 |
| Location: | 7990118 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303850 | MRPL114,mL114 | mitochondrial ribosome protein mL114, Chlamydomonas-specific | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACCGAAGTCACCTAGCAATCGATAAGC |
| Internal bar code: | GCCTCGTGAATTACGGTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 99.7173 |
| LEAP-Seq n confirming: | 2822 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGATTCATCGTGTGGGAG |
| Suggested primer 2: | AGGAGGAGGACGAAGAGGAG |