| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068581 |
| Chromosome: | chromosome 5 |
| Location: | 325249 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233300 | (1 of 1) 2.1.1.98 - Diphthine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCTCCTCCACCTCCCTCCCTCCCTCC |
| Internal bar code: | CCTAGCCTTTGATTTTAATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 85.4675 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGAGGGTGACTGGGTTG |
| Suggested primer 2: | AACTGTGTGTAGCCGTGCAG |