| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068624 |
| Chromosome: | chromosome 16 |
| Location: | 4728740 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686850 | PTA4 | (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCCCTTAAACTTTGAGTCAAAGTCGA |
| Internal bar code: | TCACAGAACTGCCGAAAAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 99.2929 |
| LEAP-Seq n confirming: | 3651 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAACGTGACTGGACTTC |
| Suggested primer 2: | TACACCCCCTAAATCCCCTC |