| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068634 |
| Chromosome: | chromosome 12 |
| Location: | 7268587 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557950 | DNJ27 | (1 of 1) K09528 - DnaJ homolog subfamily C member 8 (DNAJC8); DnaJ-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCGACGCCCTGTTTGGCTTCCGCTGAG |
| Internal bar code: | CAAGCAGCGAGGCTATTCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 99.4045 |
| LEAP-Seq n confirming: | 6176 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTTACGGCAAACGCTGT |
| Suggested primer 2: | ATAATACCACGTTGCGGAGC |