Insertion junction: LMJ.RY0402.068659_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.068659
Chromosome:chromosome 7
Location:6104993
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g355550 (1 of 4) PTHR22911:SF6 - SOLUTE CARRIER FAMILY 35 MEMBER G1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTGCATTGCCTGTTATATTGCCCCTCA
Internal bar code:GCATCTGGAACACTCGATATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:416
LEAP-Seq percent confirming:99.7596
LEAP-Seq n confirming:7470
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAAAGAACTTGGTGTCGT
Suggested primer 2:CAGTAAGCTTGGCTTGAGGG