| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068661 |
| Chromosome: | chromosome 9 |
| Location: | 3340860 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388504 | (1 of 16) IPR003903 - Ubiquitin interacting motif | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCCATCACAGCCTAGCATAAGATGCT |
| Internal bar code: | TCTATACGATGCAGATGGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 99.6823 |
| LEAP-Seq n confirming: | 17573 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTGTCAAGGCCATCTAC |
| Suggested primer 2: | TCACAAGTACCGTGAGCGAG |