| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068665 |
| Chromosome: | chromosome 7 |
| Location: | 5419210 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350350 | FAP217 | Coiled-Coil Flagellar Associated Protein 217 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTAACCCGCGGCTGCCCGGCCGCAGGT |
| Internal bar code: | GTTCAGTTTTCCGTAAAGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 99.5956 |
| LEAP-Seq n confirming: | 985 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAGTATGGACGGGGAGGG |
| Suggested primer 2: | GTATGTGCTGACTCAGGCGA |