| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068676 |
| Chromosome: | chromosome_1 |
| Location: | 3890166 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTAAGGCCTTGCGACAACCCGAAACCTGTC |
| Internal bar code: | CCATCTGGTACGGTGGTAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 99.0719 |
| LEAP-Seq n confirming: | 2135 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAATTGCTGGGTATGGT |
| Suggested primer 2: | GAAAGCGATGCTCACATGAA |