| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068690 |
| Chromosome: | chromosome 6 |
| Location: | 7160190 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297650 | UBC36 | (1 of 1) K10582 - ubiquitin-conjugating enzyme E2 Q (UBE2Q); E2 ubiquitin conjugating enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCAACCTCGTCAGGAGCTCAAGAAGTC |
| Internal bar code: | CTGTTCGGACACAGGTTGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 457 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGACCTTCGGCTACCAG |
| Suggested primer 2: | TGTATTCGTCGGGTGGGTAT |