Insertion junction: LMJ.RY0402.068710_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.068710
Chromosome:chromosome 16
Location:3471250
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g672753 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCCCAACATTTAGAACCCCCAGCAATCA
Internal bar code:GGGGTTCCTGTGGCCCGCCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:660
LEAP-Seq percent confirming:99.6104
LEAP-Seq n confirming:3068
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCAGGGCGAGAGTAACG
Suggested primer 2:GCTTTTTCGTCGCTCCTATG