| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.068713 |
| Chromosome: | chromosome 5 |
| Location: | 838548 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247900 | (1 of 13) PF00646 - F-box domain (F-box) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCACACGCCGCACCGGGCTCGTGAGC |
| Internal bar code: | GCGTGAAAGGGTCCGATCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 315 |
| LEAP-Seq percent confirming: | 92.6421 |
| LEAP-Seq n confirming: | 277 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCTTGACTAACGGCTCT |
| Suggested primer 2: | TGTCTGGAGCTCTGTGATGG |