| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068727 |
| Chromosome: | chromosome 6 |
| Location: | 5274142 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282651 | TRX16,TRL2 | (1 of 12) 1.6.5.4 - Monodehydroascorbate reductase (NADH); Thioredoxin-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTTCAGCCGCCGGCTACGGTCAACCAC |
| Internal bar code: | CGCACTGCCTACTTACCCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 63 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGTTGGAGATTTGGCT |
| Suggested primer 2: | TAAGGCGCTAACCCACAATC |