Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.068765 |
Chromosome: | chromosome 6 |
Location: | 5444030 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g284350 | CYCD3 | (1 of 1) K18810 - cyclin D1/2/4, plant (CYCD1_2_4); D-type cyclin | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCACTGACATGGAATGCAATGGTGCG |
Internal bar code: | GGCTCTAATACGACCCGACGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 787 |
LEAP-Seq percent confirming: | 84.4067 |
LEAP-Seq n confirming: | 2831 |
LEAP-Seq n nonconfirming: | 523 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATGAGGTTTCGGCTTTGCT |
Suggested primer 2: | CTGTCCGCTCCTACCTTCAG |