| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068785 |
| Chromosome: | chromosome 16 |
| Location: | 284236 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694201 | (1 of 1) PTHR12461//PTHR12461:SF46 - HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED // SUBFAMILY NOT NAMED; Cupin- Jumonji-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTTATTGCGCGCGAGGTCAGAAAGTAT |
| Internal bar code: | CAAGTATCGGTACCTTACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 99.8304 |
| LEAP-Seq n confirming: | 2355 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCACAGAGCAACGCGAC |
| Suggested primer 2: | ACTTCAATGCACACACCCAA |