| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068797 |
| Chromosome: | chromosome 1 |
| Location: | 3607280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023250 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCTCTGACACCACTCTCCCTTGCCCTC |
| Internal bar code: | AGTAGAAAAAGTATCTACAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.8128 |
| LEAP-Seq n confirming: | 3199 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGCGTGACAGATGCGTG |
| Suggested primer 2: | GCGATGTGTGTGTACAAGGG |