| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068812 |
| Chromosome: | chromosome 15 |
| Location: | 737491 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640250 | (1 of 1) K07562 - nonsense-mediated mRNA decay protein 3 (NMD3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACCTTTTGCTCGCGTGGAACTTTAGGTC |
| Internal bar code: | CATCCTCACTGATGCCCGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 99.5952 |
| LEAP-Seq n confirming: | 8120 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGATGAGGGGTGTTACG |
| Suggested primer 2: | ATTCCCTCCTCCTCCTTGAA |