Insertion junction: LMJ.RY0402.068819_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.068819
Chromosome:chromosome_7
Location:2054954
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre07.g325762 DRC11 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CACGCCCCCGCTCTCCCCGCCCTTCCACTC
Internal bar code:TTTGGGACACAATATTGGTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:445
LEAP-Seq percent confirming:99.4595
LEAP-Seq n confirming:736
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTTCCCACCACACTCTT
Suggested primer 2:ATGTGACCCCTTCTCCCTCT