| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.068837 |
| Chromosome: | chromosome 6 |
| Location: | 7971274 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303751 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGAAATCCGTGTACGTACGGTAAACAA |
| Internal bar code: | GTCCGATGTTTAGTAAACATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 99.6971 |
| LEAP-Seq n confirming: | 8229 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATCTTACCCCTCCCGTC |
| Suggested primer 2: | GTAGGTAAACGCAAGCAGGC |