Insertion junction: LMJ.RY0402.068862_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.068862
Chromosome:chromosome 13
Location:2577571
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g581000 GT90F26,GT90-26 GT90 family protein 26; (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGCATCTGGTCTGGTCCGTGGTACCGT
Internal bar code:CACGGTTTTTTGAGCATGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1050
LEAP-Seq percent confirming:99.24
LEAP-Seq n confirming:1828
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACCGTGTCTTGACATCACC
Suggested primer 2:ATCTACTACCCGCTGCGAGA