| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068870 |
| Chromosome: | chromosome 16 |
| Location: | 4457081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670800 | TFC1 | Tubulin binding cofactor A; (1 of 1) K17292 - tubulin-specific chaperone A (TBCA) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTGCTCTCAAATGGACCCCGTCGTGG |
| Internal bar code: | CGCTCGTGAAGATACCGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 99.4339 |
| LEAP-Seq n confirming: | 8606 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCATCGCAACGCTACAG |
| Suggested primer 2: | CGCTAGGTGACATTTTGGGT |