| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.068919 |
| Chromosome: | chromosome 6 |
| Location: | 7068214 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296983 | SAE2 | (1 of 1) K10685 - ubiquitin-like 1-activating enzyme E1 B (UBLE1B, SAE2, UBA2); SUMO activating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAGGATGCCGTGGGAGGGCAAGACTCC |
| Internal bar code: | AACAGACTTCGGGGGGGGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 97.9642 |
| LEAP-Seq n confirming: | 2791 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACCAAGGAGTTCAAGGA |
| Suggested primer 2: | CACACTCACTCCCCACACAC |